MICROBIOLOGY LABORATORY
The screening, isolation and characterization of microbes are critical for success in biotechnology discipline. Microbes provide with rich variety (including beneficial and pathogenic microbes) of structure, forms, and compounds that has been efficiently utilized in many functional domains. At TSBS, the Microbiology Laboratory caters to the biotechnology and applied microbiology courses. The following are the list of instruments in the microbiology facility-
LIST OF MAJOR EQUIPMENTS
| Sl No | Name of the Equipment | Quantity | Make | Model | Specification |
| 1. | LAMINAR AIR FLOW BENCH | 1 | SATYAM | NA | Size- 3’x2‘x2’ |
| 2. | BINOCULAR RESEARCH MICROSCOPE | 5 | OLYMPUS MAGNUS | MLXB | Microscope stand with quadruple nose piece low drive mechanical stage, constant voltage illuminator base. Binocular inclined observation head, mechanical stage with right hand low drive controls, sub-stage condensers, blue filter Achromatic objective 4X, 10X, 40X, 100X oil immersion |
| 3. | LABORATORY AUTOCLAVE | 1 | YORKO | VSI-403 | Outer wall- crc sheet finished with stoving enamel paint or polished stainless steel. Inner chamber- leak proof, steam tight, hydraulically tested at double the operating steam pressure, made of heavy gauge stainless steel
Stainless steel steam jacket, lid, Neoprene rubber gasket, steam pressure gauge fitted to the unit to indicate steam pressure. Safety valve to release pressure if exceeds safety limit |
| 4. | HOT AIR OVEN | 2 | YORCO | YSI 431D | Outer wall- CRCA sheets duly powder coated.
Inner chamber- Aluminium/stainless steel with ribs on walls. Insulation- 75mm thick, high grade glass/mineral wool. Heating element- nichrome wire, adjustable height. Temperature .range : 50o C to 250o C |
| 5. | INCUBATOR/ WATER BATH SHAKER | 1 | RIVOTEK | 50111003 | Shaking Speed 20-200 Orbital strokes per min. permanent Magnet Geared DC motor with 25mm dia motion |
| 6. | DIGITAL TOP LOADING ELECTRONIC PRECISION BALANCE | 1 | CITIZEN | C4-300 | Capacity- 300gms |
| 7. | DIGITAL pH Meter | 2 | SYSTRONICS | 335 | —
|
| 8. | DIGITAL COLONY COUNTER | 1 | HIMEDIA | LA660/140/0923 | — |
| 9. | AUTO PIPETTES (Variable Volume) |
5 | VARIOUS VOLUMES | — | |
| 10. | BOD INCUBATOR | 1 | SATYAM | — | Voltage required- 220/225 volts, single phase 50 cycles.
Cooling- 4/50C below the ambient temperature High- 4/50C above the ambient temperature upto 500C |
| SL NO | TITLE OF EXPERIMENT |
| 1 | STERILIZATION AND PREPARATION OF CULTURE MEDIA |
| 2 | CULTURE OF BACTERIA FROM WATER AND SOIL |
| 3 | IDENTIFICATION AND OBSERVATION OF BACTERIA BY GRAM STAINING |
| 4 | DETERMINATION OF BACTERIA SENSITIVITY TO ANTIBIOTICS |
| 5 | DETERMINATION OF OXYGEN CONSUMPTION IN MICROBES |
| 6 | ISOLATION AND MAINTENANCE OF ORGANISMS BY PLATING, STREAKING, SERIAL DILUTION METHOD, SLANTS AND STAB |
| 7 | PURE CULTURE TECHNIQUES |
| 8 | GROWTH CURVE, MEASUREMENTS OF BACTERIAL POPULATIONS AND GENERATION TIME BY TURBIDOMETRY AND CELL COUNTING |
| 9 | Staining Techniques for bacteria & fungi. |
| 10 | Micrometry(spores, conidiophores, conidia measurements) |
| 11 | Effect of limperature, inubation period & PH on growth of fungi & bacteria |
| 12 | Study of Thermal Death point(TDP) of bacteria |
| 13 | Biochemical Analysis(IMVIC test, TSI, KIA, Urease, Oxidase) |
| 14 | Estimation of dissolved O2(OO), CO2 Content, total chloride content, total alkalinity content, total audity content, total hardness & total chlorine content of water samples. |
| 15 | Isolation and identification of Rhizobium species from root nodules and rhizosphenic soil. |
| 16 | Wine and alcohol preparation. |
| Beyond Syllabus Experiments | |
| 1 | Recombinant Protein Expression in E. coli |
| 2 | Application of REPLICA technical for isolation of antibiotic sensitive and resistant bacteria |
| 3 | Estimation of Sulphate-reducing bacterial count using MPN method |
| 4 | Isolation of extracellular beta-glucan from different fungal strains |