MOLECULAR BIOLOGY LABORATORY
The road to next-generation drugs be it protein or DNA-based bases its fundamentals on three modules:
DNA–>RNA–>Proteins.
At TSBS Molecular Biology facility, experiments are conducted to understand the three different modules through qualitative and quantitative assays.
| SL NO | NAME OF THE INSTRUMENT | QUANTITY | MAKE | MODEL | SPECIFICATION |
| 1 | THERMOCYCLER(PCR DEVICE) | 1 | Eppendorf (Germany) |
Mastercycler Personal | -Temperature control is carried out with Peltier elements which enable extremely rapid changes in temperature of upto 30C/sec. this allows the temp of the samples to be controlled extremely rapidly between 40C & 990C.- A special tube control mode has been developed for temp.controlling samples in various different tubes with various different filling volumes.
– The thermo block has been constructed as a universal block which can accommodate micro titer plates in the 5X5 format, 25 Eppendorf PCR test tubes of 0.2ml, 16 thin walled Eppendorf PCR test tubes of 0.5ml without the block having to be exchanged. – To prevent the formation of condensation, the device has a heated lead which has been perfectly tailored to suit all tubes. – The Mastercycler personal is an authorized cycler licensed by Perkin- Elmer Corp. |
| 2 | SPECTROPHOTOMETER UV- VISIBLE |
1 | SYSTRONICS | Type-118 | Complete mirror optics with 1200 grooves/mm grating Czerny-Turner mount.Wavelength length- 200-900 nm.
Readout- digital counter, 0.2 nm increments. Accuracy- +_0.5nm. Spectral bandwidth- better than 200-900 nm. Photometric range- 0-2.000 Abs, 0-1999 concentration units, 0-100.0/transmittance, Dimensions- 800mmX 400mmX 300mm, power req. 230V, 50Hz |
| 3 | DIGITAL TOP LOADING ELECTRONIC PRECISION BALANCE | 1 | CITIZEN | C4-300 | — |
| 4 | DIGITAL PH METER | 3 | SYSTRONICS | MODEL-335 | |
| 5 | HORIZONTAL SLAB GEL WITH ELECTROPHORESIS POWER PACK | 10 | GENEI | DIGITAL PS 500 | (Agarose gel electrophoresis of DNA) |
| 6 | VERTICAL SLAB GEL WITH POWER PACK FOR ELECTROPHORESIS | 10 | GENEI | MINI GEL SYSTEM | (for SDS-PAGE of proteins) |
| 7 | UV TRANS ILLUMINATOR | 1 | GENEI | GENEI T-20 | To visualize DNA bands stained with a fluorescent dye |
| 8 | TABLE TOP HIGH SPEED CENTRIFUGE | 1 | GENEI | SLM MCF 14K | Upto 20000 rpm |
| 9 | AUTO PIPETTES (Variable Volume) |
15 | VARIOUS COMPANIES | — | |
| 10 | ELISA READER | 1 | SYSTRONICS | MODEL 642 | — |
| 11 | ORBITAL SHAKER | 1 | RIVOTEK | 5008/004 | |
| 12 | HOMOGENIZER | 1 | REMI | RQ-127A | 8000 RPM, 220/230V, 1.1AMPS, 50 CYCLES |
| 13 | GEL ROCKER | 1 | GENEI | ||
| 14 | HIGH SPEED REFRIGERATED CENTRIFUGE | 1 | ELTEK | RC4100D | Maximum speed- 20000 rpm, maximum centrifugal force- 34500g, max.tube size- 100ml, max.capacity- 400ml.Lowest temp. -200C. weight- 80 kg, electrical load at230V, 50Hz, 1.75KVA |
| SL NO | NAME OF THE EXPERIMENT |
| 1 | SDS PAGE |
| 2 | EXTRACTION OF GENOMIC DNA FROM PLANT OR ANIMAL |
| 3 | AGAROSE GEL ELECTROPHORESIS |
| 4 | SOUTHERN BLOTTING |
| 5 | NORTHERN BLOTTING |
| 6 | WESTERN BLOTTING |
| 7 | ISOLATION OF RNA/mRNA |
| 8 | ELISA |
| 9 | ISOLATION AND PURIFICATION OF PLASMID |
| 10 | RESTRICTION DIGESTION AND MAPPING OF PLASMID |
| 11 | PREPARATION OF COMPETENT CELLS |
| 12 | BACTERIAL TRANSFORMATION |
| 13 | RESTRICTION DIGESTION OF LAMBDA DNA |
| 14 | PCR/RFLP/RAPD |
| Beyond Syllabus Experiments | |
| 1 | Analysis of Amylases by SDS-PAGE and Western transfer |
| 2 | Enzyme Kinetics and Inhibition using an ELISA Reader |
| 3 | Isolation of nuclear, chloroplast, and mitochondrial DNA from plants |
| 4 | Protein structure using NATIVE-PAGE and conformation using SDS-PAGE (reducing and non-reducing conditions) |